Yes, that is a real cuttlefish and it is trained to wear 3-D glasses.
"Cuttlefish catch a meal by deploying their tentacles and, to be successful in their strike, cuttlefish must compute depth to position themselves at the correct distance from the prey. If they are too close, the prey may be spooked and escape; too far and the tentacles will not reach.
To test how the cuttlefish brain computes distance to an object, the team trained cuttlefish to wear 3-D glasses and strike at images of two walking shrimp, each a different color displayed on a computer screen at the Marine Biological Laboratory in Woods Hole, Mass."
Video at the link:
https://phys.org/news/2020-01-d-movies-reveal-cuttlefish-distance.html
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